| p53 |
0 = absence and 3 = maximum score in both cases. |
| p16 When present, the intensity in basal layer and in supra-basal layers was considered independently as a semi-quantitative assessment |
0 = absence and 3 = maximum score in both cases. |
| Bcl2 Extension of the staining was considered as diffuse or focal in one variable and affecting only the 1/3 basal epidermis or 2/3 of the epidermis, both as qualitative traits. |
0 = absence and 3 = maximum score. |
| Ki67 |
Intensity Ki67 staining was considered normal (score 0) when 1 basal epidermal cell was positive every 10 or more basal cells, score 1 when more than 1 basal cells were positive in less than 10 basal cells, score 2 when consecutive basal cells were positive and score 3 when consecutive basal and supra-basal cells were positive. |
| p27 Staining was evaluated in the basal layer, supra-basal layer and superficial epidermis as a semi-quantitative assessment |
0 = absence and 3 = maximum score in both cases. |
| p21 nuclear staining were evaluated in the 1/3 basal layer and in the 2/3 supra-basal layer as a semi-quantitative quantification in each case |
0 (absence), 1 (more the 10% of nucleus and less than 50% of the nucleus), 2 (more than 50% of nucleus and less than 90% of the nucleus), to 3 (more than 90% of nucleus). |
| PCNA nuclear staining were evaluated in the 1/3 basal layer and in the 2/3 supra-basal layer as a semi-quantitative quantification in each case and scored from |
0 (absence), 1 (more the 10% of nucleus and less than 50% of the nucleus), 2 (more than 50% of nucleus and less than 90% of the nucleus), to 3 (more than 90% of nucleus). |
