Figure 1: Illustration of the experimental design. After 28 days of the implantation of the B16F10 melanoma cells, the animals were treated with drug and radio frequency (RF) with delivery interval of 72 hours, for 20 days. The treatment consisted in thermal ablation induced by fractionated RF. (a,b) illustration of the application of the tumor cells subcutaneously in mice. (c,d) Figures illustrating the application of the drug and RF; (c) First it was applied to RF and then the drug; (d) First the drug was applied and then the RF. (e, f) Action of RF in the skin; the machine promote several small ablations points in skin surface causing several penetrations channels into to deep epidermis (arrow); (e) Several columns of RF in the skin causing micro ablation points; (f) Photomicroscopy of the epidermis absence in specific points forming penetrations points.