Day Stressing factor Duration 1 Water deprivation 24 h 2 Food deprivation 24 h 3 Isolation 24 h 4 Isolation 24 h 5 Isolation 24 h 6 Flashing light 3 h 7 Food deprivation 24 h 8 Forced swimming 10 min 9 Wet litter 1 h 10 Water deprivation 24 h 11 No stress - 12 No stress - 13 Wet litter 4°C 2 h 14 Flashing light 2 h 1/2 15 Food deprivation 24 h 16 Forced swimming 15 min 17 Isolation 24 h 18 Isolation 24 h 19 Isolation 24 h 20 Water deprivation 24 h 21 Food deprivation 24 h 22 Flashing light 3 h 23 Wet litter 1 h 24 Isolation 24 h 25 Isolation 24 h 26 Wet litter 4°C 1 h 1/2 27 Forced swimming 10 min 28 Flashing light 3 h 1/2 29 No stress - 30 Food deprivation 24 h 31 Wet litter 1 h 32 Flashing light 2 h 33 Water deprivation 24 h 34 Wet litter 4°C 2 h 35 Forced swimming 10 min 36 Isolation 24 h 37 Isolation 24 h 38 No stress - 39 Flashing light 3 h 40 Forced swimming 10 min

Table 1: Program of stressors and duration applied every day.