Figure 1: Effects of IgM anti-GalNAc-GD1a mAb on spontaneousmuscle action potentials in spinal cord–muscle co-cultured cells.(A) Blockade of spontaneous muscle action potentials by IgM anti-GalNAc-GD1a mAb (1:100 dilution, 10 μL) in spinal cord–muscleco-cultured cells. This blockade was fully reversed after 4 min bywashing with the co-culture solution. (B) Time course of thefrequency of spontaneous muscle action potentials after addition ofIgM anti-GalNAc-GD1a mAb (1:100 dilution, 10 μL). IgM anti-GalNAc-GD1a mAb decreased the spontaneous muscle actionpotentials in the spinal cord–muscle co-cultured cells. Afterwashing out the IgM anti-GalNAc-GD1a mAb, recovery frominhibition was observed. The number of spontaneous muscle actionpotentials was measured every 5 sec. (C) Inhibition rate of thespontaneous muscle action potentials following addition of IgManti-GalNAc-GD1a mAb (1:100 dilution). Data are the mean ±SEM. n = 4 for IgM anti-GalNAc-GD1a mAb (1:100 dilution, 10μL). **P < 0.01 versus control.