Figure 4: Immunostaining with IgM anti-GalNAc-GD1a mAb andcalcium channel antibody in cross-sections and longitudinalsections of rat hemi-diaphragms. (A) Staining with IgM anti-GalNAc-GD1a mAb (green) overlapped with the postsynapticacetylcholine receptor cluster marker α-bungarotoxin (α-BuTx,red) and anti-Cav2.1 (α1A) antibody (P/Q-type calcium channel;blue) at the nerve terminal. (B) Staining with IgM anti-GalNAc-GD1a mAb (green) was not overlapped with anti-Cav2.2 (α1B)antibody (N-type calcium channel; blue). (C) Staining with IgManti-GalNAc-GD1a mAb (green) was not overlapped with anti-Cav1.2 (α1C) antibody (L-type calcium channel; blue). The barsrepresent 5 μm.