Figure 1: Inducible expression of low levels of hAβPP and hBACE1 in Drosophila. A: RU486-induced expression of hAβPP/hBACE1 in the neuronal tissues of Drosophila. B-C: Elav-GeneSwitch driver induces low-level hAβPP/hBACE1 expression. High-level expression of hAβPP/hBACE1 was driven by Elav-Gal4. RU486 inducible expression of hAβPP/hBACE1 was driven by Elav-GS. Head lysates were prepared from flies expressing hAβPP/hBACE1, driven by Elav-Gal4 or Elav- GS, and from control lines not expressing hAβPP/hBACE1. These lysates were subjected to Western blot with anti-AβPP antibody and anti-β-actin antibody. For sAβPPβ (cleavage product of hAβPP by hBACE1) assay, lysate samples were subjected to serial dilutions with Alphalisa buffer, and sAβPPβ levels were detected by PerkinElmer AlphaLISA kit.