Figure 2: Co-immunostaining of GFP-PrPC and endogenous PrPC with microtubules (MTs). Anti-tubulin DM1A (Panels A and E) and anti-PrP K3 (Panels B and F) detects PrPC along with MTs in the presence (Panels G-H; merged) or absence (Panels C-D; merged) of NGF. Panels D and H shows magnified images. In this experimental condition, anti-PrP polyclonal antibody K3 recognized endogenous and GFP-fused PrPC because it was raised against the PrP peptides (amino acid residues 76–90 in mouse PrP). Scale bars; 15 μm.