Figure 1: Verification of adult keratocytes in KGM. A) phase-contrast image of keratocytes after four passages in KGM. Original magnification 4x. B) Gene expression of ALDH3A1, CDH5, and keratocan in passage 4 as demonstrated by RT-PCR. Keratocytes had negative expression of α-SMA. Lung fibroblasts treated by TGF-β were used as positive control for α-SMA expression.