| Fixation and anesthesia |
Description |
Human resources |
No of Birds |
| Manual restraint of unsedated bird |
The bird´s head held gently between thumb and index finger of one hand, while the extremities held among the fingers of the other hand. Eyelid kept opened with a moistened cotton-tip applicator. A soft towel/tissue was rolled to the birds, to prevent plumage damage. |
1) OCT operator
2)Bird handler
3) Person holding the eyelid open |
14 |
| Non-sedated bird on a fixation plate |
Stabilization plate: a light, wooden frame (length: 38 cm, width: 25 cm) with three horizontal movable wooden bars to support the bird´s dorsum. The head/neck held by an assistant to correct positioning. The body was strapped on with elastic bands. Legs stretched on two soft cushioned modified wooden processes of the frame (Figure 1). Eyelid kept opened with a moistened cotton-tip applicator. |
1) OCT operator
2) Bird handler/ Person holding the fixation plate upright
3) Person holding the eyelid open |
1 |
| Manual restraint of sedated bird |
Manual restraint as in the first method described. Eyelid kept opened with a moistened cotton-tip applicator.
Sedation: Intranasal midazolam (2-6 mg/kg, Midazolam-ratiopharm® 5mg/1ml, Ratiopharm GmbH, Germany) reversed by intranasal flumazenil (0,05 mg/kg, Anexate, vial 0,5 mg/ml, Roche Ltd, UK) or combination of intranasal midazolam and intramuscular butorphanol (1 mg/kg, Toburgesic, 10 mg/ml, Fort Dogde, USA) reversed by intranasal flumazenil (0,05 mg/kg, Anexate, vial 0,5 mg/ml, Roche Ltd, UK). |
1) OCT operator
2) Bird handler
3) Sedationmonitoring and holding the eyelid open |
5 |
| Sedated bird
on a fixation plate |
Sedation as in the third method described.
Stabilization plate as in the second method described. |
1) OCT operator
2) Bird handler/ Person holding the fixation plate upright, sedation monitoring and holding the eyelid open |
5 |
| Manual restraint of anesthetised bird |
Manual restraint as in the first method described
Anesthesia: Facemask and standard isoflurane (induction 5%, maintenance 1,5-2,5%, Forane USP, Baxter Healthcare Corporation, USA) and oxygen (induction 2 l/kg, maintenance 0,8-1l/kg flow) concentrations. Intubation and basic anesthetic monitoring by vet (heart rate, respiratory rate, body temperature, ventilation). |
1) OCT operator
2) Bird handler
3) Anaesthesia and intubation monitoring and holding the eyelid open |
6 |
| Anesthetised bird on a fixation plate |
Anesthesia as in the fifth method described.
Fixation plate as in the second method described. |
1) OCT operator
2) Bird handler/ Person holding the fixation plate upright and holding the eyelid open
3) Anaesthesia and intubation monitoring |
8 |
