Figure 6: Terminal deoxynucleotidyl transferase mediated dUTP Nick End Labeling assay (TUNEL) staining to detect apoptotic and necrotic nuclei. (A) Non-injected control and (B) BSS-injected control eyes have minimal cell death detectable by TUNEL labeling. (C) Subretinal polymer injected eyes have apoptotic nuclei mainly in the outer nuclear layer at the needle entry site and no TUNEL positive nuclei were detected outside the injection site (D). Nuclei are stained with DAPI (E-H).