Figure 8: PEDF signaling protects RGCs from pressure-induced apoptosis. A. Graphical representation of the percentage TUNEL+RGCs treated with vehicle or the PEDF-R specific inhibitor aglistatin (50 μM, 100 μM, 200 μM, 400 μM) and exposed to ambient or elevated pressure for 48 hours. Asterisks denote statistical significance (p<0.05) between vehicle and drug treatments within each pressure condition. B. Graphical representation of the same data in (A) depicted as the percentage change in TUNEL+RGCs between ambient and elevated pressure within each drug treatment. Asterisks denote statistical significance between ambient and elevated pressure within each drug treatment (p<0.05). C. Graphical representation of the percentage of TUNEL+RGCs treated with vehicle, 50ng/ml recombinant PEDF (rPEDF) or 100 ng/ml rPEDF and exposed to ambient or elevated pressure for 48 hours. Asterisks denote statistical significance (p<0.05).