Primary antibodies used for immunohistochemistry

Target	Antibody name	Concentration used	Vendor	Catalog #
PEDF	Mouse anti-human PEDF	20 μg/ml	Abcam (Cambridge, MA)	115489
PEDF-R	Goat anti-human PEDF-R/PNPLA2	20 μg/ml	R&D Systems (Minneapolis, MN)	AF5387
PEDF-R	Goat anti-human Adipose Triglyceride Lipase	2.5 μg/ml	Abcam (Cambridge, MA)	85858
Retinal ganglion cell (RGC)	Mouse anti-mammalian SMI-31 (phosphorylated neurofilaments)	1 μg/ml	Covance (Princeton, NJ)	SMI-31R
Retinal ganglion cell (RGC)	Rabbit anti-mammalian Gamma Synuclein	10 μg/ml	Abcam (Cambridge, MA)	55424
Müller Cells	Goat anti-human Glutamine Synthetase	4 μg/ml	Santa Cruz Biotechnologies (Santa Cruz, CA)	Sc-6640
	Rabbit anti-human Glutamine Synthetase	4 μg/ml	Santa Cruz Biotechnologies (Santa Cruz, CA)	Sc-6640-R

Table 1: Primary antibodies used for immunohistochemistry and immunocytochemistry. Primary antibodies, sources, and concentrations used for detection of PEDF, PEDF-R and labeling of RGCs and Müller cells in murine retina.