Figure 2: Activation of MAPKs and changes of IOP in DBA/2J mice. Protein extracts were resolved by SDS-PAGE and immunoblotted with antibodies against (A) phospho-p38 MAPK, total-p38 MAPK (38 kDa), (B) phospho-ERK 1/2, total-ERK 1/2 (44 and 42 kDa). (C) ERK 1 and ERK 2 values were separately plotted to underline their different modulation. MAPK activation was evaluated as the ratio between phosphorylated and total protein. Data are reported as mean ± SEM (Standard Error of the Mean) and expressed as percent of control (3 month DBA/2J mice; at least three retinas/group were processed); *p<0.05.