No isolates Date of isolement Unit of isolementa Clusters of RAPD typingb Clusters of PFGE typingc Antibiogram patterns in clusterd Integron1 detection Accenssion number of abeB gene 1 06.01.04 ICU F1 1 a1 820bp ST IV 2 13.10.04 Surgery F1 1 a1 820pb+2,2Kb ST IV 3 24.07.05 ICU F1 1 a1 (-) ST IV 4 27.10.04 ICU C1 2 a1 (-) ST III 5 20.09.04 ICU C1 2 a1 2,2Kb ST III 11 15.06.04 ICU C1 2 d1 (-) ST III 13 06.06.05 CVU C1 2 e1 820pb ST III 15 02.04.05 other unit C1 2 e1 2.2 Kb ST III 29 13.12.05 other unit C1 2 k1 (-) ST III 6 19.10.05 ICU G1 3 b1 (-) ST V 9 23.10.05 ICU G1 3 b1 2,2 Kb ST V 7 25.10.05 other unit G1 3 b1 2,2 Kb ST V 10 30.01.05 ICU G1 3 c1 (-) ST V 23 22.01.05 ICU G1 3 g1 (-) ST V 27 12.04.04 other unit G1 3 i1 (-) ST V 28 10.10.05 ICU G1 3 j1 (-) ST V 12 10.01.05 CVU G1 3 e1 (-) ST V 16 13.09.04 ICU G1 3 e1 820pb ST V 14 23.03.05 ICU B1 4 e1 820pb AF 390885 25 22.01.04 ICU B1 4 h1 (-) AF 390885 30 27.08.04 Surgery B1 4 k1 (-) AF 390885 17 17.08.05 ICU A1 e1 (-) ST II 18 02.06.05 CVU A1 5 e1 (-) ST II 19 08.02.05 ICU A1 5 f1 (-) ST II 21 24.04.05 ICU A1 5 f1 (-) ST II 22 24.09.05 others unit A1 5 f1 (-) ST II 20 14.05.05 other unit E1 6 f1 (-) ST I 24 31.05.05 Other unit E1 6 g1 (-) ST I 26 07.10.05 other unit E1 6 h1 (-) ST I 8 06.09.05 CVU E1 6 b1 (-) ST I aICU: intensive care unit ; CVU: cardiovascular unit bDefined as isolates belonging to the same genomic species that have an RAPD similarity >90% cDefined as isolates belonging to the same genomic species that have an PFGEsimilarity >90% dAntibiogram patterns as defined in the table 2

Table 4: Summary of the molecular and antibiogram clusters of Acinectobacter baumannii isolates from the Tunisian hospital.