Figure 3: Wolbachia are located in hypodermal cords in infective third stage larvae (L3) of Brugia malayi by ISH. The top panel shows ISH results obtained with a 16S rRNA probe. The middle panel shows ISH labeling merged with cell membrane staining using wheat germ agglutinin (Alexa fluor 633 conjugated); the bottom panel shows ISH results merged with DAPI staining. The color of ISH staining has been replaced by red to increase contrast before merging with other layers. (A) Longitudinal section to show Wolbachia localization along the lateral cord; (B) cross section to show Wolbachia localized in two lateral cords; (C) cross section showing Wolbachia only present in one lateral cord. Arrows point to lateral cords, arrow heads point to dorsal and ventral cords; scale bar: 25 μm.