Table 2

Growth conditions		Biofilm production (OD570)
Growth conditions		Negative	A19008	A19010	CECT 4638	CAIM 1797	JM089
Culture medium
	BHIB	0.10 ± 0.03	2.23^a ± 0.28	2.01^a ± 0.39	2.21^a ± 0.37	1.31^a ± 0.28	2.78^a ± 0.20
	LB	0.09 ± 0.03	0.83^b ± 0.19	0.82^b ± 0.16	0.74^b ± 0.26	0.48^b ± 0.11	0.49^b ± 0.11
	M63	0.09 ± 0.02	0.12^c ± 0.01	0.11^c ± 0.01	0.10^c ± 0.01	0.14^c ± 0.02	0.16^c ± 0.02
	NB	0.13 ± 0.13	0.30^c ± 0.10	0.24^c ± 0.03	0.33^c ± 0.12	0.19^c ± 0.01	0.46^b ± 0.16
	TSB	0.10 ± 0.02	2.73^d ± 0.14	2.00^a ± 0.41	2.03^a ± 0.24	2.44^d ± 0.15	overflow
Culture time (h)
	0	0.22 ± 0.04	0.21^a ± 0.02	0.25^a ± 0.05	0.24^a ± 0.02	0.22^a ± 0.04	0.23^a ± 0.02
	6	0.12 ± 0.01	0.11^a ± 0.01	0.10^a ± 0.00	0.10^a± 0.00	0.11^a ± 0.00	0.32^a ± 0.05
	12	0.12 ± 0.01	0.14^a ± 0.02	0.12^a ± 0.01	0.12^a ± 0.01	0.12^a ± 0.01	2.05^b ± 0.68
	18	0.11 ± 0.03	0.36^ab ± 0.04	0.12^a ± 0.04	0.10^a ± 0.28	1.29^b ± 0.01	overflow
	24	0.12 ± 0.01	1.62^c ± 0.03	0.60^b ± 0.14	1.13^b ± 0.09	1.54^b ± 0.41	overflow
	36	0.11 ± 0.01	0.31^ab ± 0.06	0.34^ac ± 0.10	0.85^ab ± 0.14	0.34^ab ± 0.20	overflow
	48	0.10 ± 0.01	0.22^a± 0.17	0.27^a ± 0.05	0.86^ab ± 0.08	0.30^ac ± 0.15	overflow
NaCl concentration (%)
	0.5	0.07 ± 0.01	0.08^a ± 0.00	0.07^a ± 0.04	0.06^a ± 0.00	0.06^a ± 0.00	1.83^a ± 0.09
	1	0.07 ± 0.01	0.08^a ± 0.00	0.08^a ± 0.00	0.11^a ± 0.00	0.08^a ± 0.06	2.33^b ± 0.11
	2	0.07 ± 0.01	1.43^b ± 0.17	0.75^b ± 0.32	1.32^b ± 0.26	0.59^b ± 0.26	overflow
	3	0.07 ± 0.01	0.07^a ± 0.01	0.07^a ± 0.01	0.07^a ± 0.00	0.07^a ± 0.02	overflow
	4	0.07 ± 0.01	0.14^a ± 0.03	0.11^a ± 0.02	0.08^a ± 0.01	0.09^a ± 0.01	0.09^c ± 0.01
Glucose concentration (%)
	0	0.07 ± 0.01	1.99^a ± 0.15	0.70^a± 0.14	0.99^a ± 0.18	1.57^a ± 0.24	overflow
	0.05	0.10 ± 0.05	1.42^b ± 0.20	1.28^b± 0.05	0.51^ab ± 0.05	1.32^a ± 0.17	2.70^a ± 0.05
	0.20	0.12 ± 0.34	1.39^b ± 0.15	1.41^b ± 0.34	0.36^b ± 0.23	1.69^a ± 0.27	2.48^a ± 0.15
	0.50	0.11 ± 0.03	overflow	overflow	overflow	overflow	overflow
	0.75	0.07 ± 0.01	1.10^bc ± 0.04	1.28^b ± 0.11	1.14^c ± 0.01	1.10^b ± 0.02	1.13^b ± 0.04
	1	0.10 ± 0.03	1.23^b ± 0.06	1.20^b ± 0.13	1.12^c ± 0.32	1.09^b ± 0.09	overflow
	1.50	0.12 ± 0.03	1.35^b ± 0.02	0.40^c ± 0.03	0.20^b ± 0.22	0.65^c ± 0.14	1.34^b ± 0.12

BHIB: Brain-Heart Infusion Broth (BBL, USA); LB: Luria-Bertani broth; M63 was made as follows: 68 g l^-1 KH₂PO₄, 10 g l^-1 (NH₄)₂SO₄, 0.246 g l^-1MgSO₄ • 7H₂O, 2.5 mg l^-1FeSO₄ • 7H₂O, 2 g l^-1 D–glucose; NB: Nitrate Broth (Difco, USA); TSB: Tryptic Soy Broth (Bacto, USA). All the above media were supplemented with final 2% NaCl. Data are expressed as mean ± SD.abcd Means in the same strain with different superscript are significantly different (p < 0.05) between growth conditions for one treatment (e.g medium).

Table 2: Biofilm production of Vibrio scophthalmi under the different growth conditions.