Enzyme Cut Buffer Thermal Digestion Inactivation TaqI 5’ …TGGA … 3’ 3’ …AGCT … 5’ Unique 65°C 2/0L  of EDTA 0/5M BSURI 5’ …GCCC … 3’ 3’ …CCCG … 5’ R 37°C 80°C for 20 min RSaI 5’ …GTAC … 3’ 3’ …CATG … 5’ Tango 37°C 65°C for 20 min AluI 5’ …AGCT … 3’ 3’ …TCGA … 5’ Tango 37°C 65°C for 20 min

Table 1: Twenty microlitres of amplified DNA from the PCR reaction were subjected to restriction enzymes digestion using TaqI, BSURT, AIUI, and RsaI. According to manufacturer’s instructions (Promega, USA) and visualised by agarose gel electrophoresis.