Figure 4: Nuclear proteins binding to the R9 sequences (-226 to -218 bp) of the EhPgp1 gene core promoter. Top, EMSA, lane 1, free probe; lane 2, no competitor; lane 3, unspecific competitor (UC, poly d(I-C)); lane 4, specific competitor (SC, Pgp1-226/218R3 oligonucleotide); lanes 5 to 7, specific competitor Pgp1-226/218R2 and Pgp1-226/218R1 (containing two or one copies of the R9(2) sequence respectively); and Pgp1-234/197 (wild type EhPgp1 promoter sequence) respectively. Arrowheads, specific complexes formed with NE. Bottom, sequences of oligonucleotides used as specific competitors in EMSA assays. The nucleotides in bold correspond to the R9 motifs.