| Technology |
Approach |
Read length |
Bases/Run |
Company and Web Addresses |
|
Automated Sanger sequencer ABI3730xl |
Synthesis in the presence of dye terminators |
Up to 900 bp |
96 kb |
Applied Biosystems www.appliedbyosystems.com |
| 454/Roche FLX system |
Pyrosequencing on solid support |
200-300 bp |
80-120 Mb |
Roche Applied Science www.roche-applied-science.com |
| Illumina/Solexa |
Sequencing by synthesis with reversible terminators |
30-40 bp |
1 Gb |
Illumina, Inc.
www.illumina.com |
| ABI/SOLiD |
Massively parallel sequencing by ligation |
Up to 75 bp |
1-3 Gb |
Applied Biosystems www.appliedbyosystems.com |
| SMRT |
Single molecule real-time sequencing |
2,200 bp on average |
120 Mb |
Pacific Bio Sciences www.pacificbiosciences.com |
| Ion Torrent |
Massively parallel semiconductor sequencing |
100 bp on average |
Up to 10 Gb |
Life Technologies www.invitrogen.com |
