Isolate Amplified gene Forward PCR primer sequence (5"-3") Reverse PCR primer sequence (5"-3) E. coli ST TTTATTTCTGTATTG TCTTT ATTACAACACAGTTCACAG   LT AGCAGGTTTCCCACCGGATCACCA GTGCTCAGATTCTGGGTCT Salmonella sp. fimA CCTTTCTCCATCGTCCTGAA TGGTGTTATCTGCCTGACCA St. aureus nuc TCAGCAAATGCATCACAAACAG CGTAAATGCACTTGCTTCAGG   mecA GGGATCATAGCGTCATTATTC AACGATTGTGACACGATAGC   16S rRNA GTGCCAGCAGCCGCGGTAA AGACCCGGGAACGTATTCAC

Table 1: PCR primer sequences used for identification of E. coli, Salmonella spp. and St.aureus isolated from feces of calves suffering from diarrhea