Culture media utilized Detection
E. coli Salmonella spp. St. aureus
Nutrient agar Smooth, rounded, white to grayish colony with unusual putrid odor Small, circular and smooth colonies Growth of circular, small, smooth, convex, and golden yellowish colonies
Blood agar Demonstrate haemolysis Demonstrate haemolysis Demonstrate haemolysis
Mac Conkey agar Rosy pink lactose fermenter colonies. Colorless, pale, translucent colony. No growth (-)
Salmonella, Shigella (SS) agar Pink color colony Translucent colorless smooth colony No growth (-)
Staphyloccous Agar no. 1 0 No growth (-) No growth (-) Yellow colonies
Eosin-Methylene Blue (EMB) agar Moist circular colonies with dark centers yellow, green metallic sheen No growth (-) No growth (-)
Table 3: Identification of isolated microbial pathogens by cultural properties