Dilution range (μg/ml) Mean MIC MIC 50 MIC 90 Average start time (min)
Ws All isolates A B C A B C A B C  
Silver 10 nm 0.039-160 0.625-5 L25-10 0.625-5 2.5 5 1.25 5 10 5 5
Silver 20 nm 0.039-160 0.312- 0.31- 0.625- 1.25 1.25 1.25 2.5 tr) 2.5 1
Gold 10 nm 0.039-160 2.5 2.5 2.5 5 10 5 20 20 10 30
Gold 20 nm 0.039-160 2.5-20 5-20 5-10 5 5 5 10 if") 10 15
Copper 10 nm 0.039-160 2.5-10 1.25-5 2.5-10 10 10 10 20 20 20 15
Copper 20 nm 0.039-160 5-20 2.5-20 2.5-20 5 5 2.5 10 10 10 5
Table 4: Determination of MIC and onset time of action of the AgNPs, AuNPs and CuNPs against E. coli, S. typhyimurium and St. aureus isolated from feces of calves suffering from diarrhea. A = E. coli, B = Salmonella, C = St. aureus