Locus Primer Primer sequence
Primary PCR
msp 1 msp1-P1 5′ -CACATGAAAGTTATCAAGAACTTGTC-3′
  msp1-P2 5′ -GTACGTCTAATTCATTTGCAC-3′
msp 2 msp2-1 5′ -ATGAAGGTAATTAAAACATTGTCTATTATA-3′
  msp2-4 5′ -ATATGGCAAAAGATAAAACAAGTG-3′
Secondary PCR
msp 1 K1-K1 5′ -GAAATTACTACAAAAGGTGCAAGTG-3′
  K1-K2 5′ -AGATGAAGTATTTGAACGAGGTAAAGTG-3′
  MAD20-M1 5′ -GAACAAGTCGAACAGCTGTTA-3′
  MAD20-M2 5′ -TGAATTATCTGAAGGATTTGTACGTCTTGA-3′
  R033-R1 5′ -GCAAATACTCAAGTTGTTGCAAAGC-3′
  R033-R2 5′ -AGGATTTGCAGCACCTGGAGATCT-3′
msp 2 3D7-A1 5′ -GCAGAAAGTAAGCCTTCTACTGGTGCT-3′
  3D7-A2 5′ -GATTTGTTTCGGCATTATTATGA-3′
  FC27-B1 5′ -GCAAATGAAGGTTCTAATACTAATAG-3′
  FC27-B2 5′ -GCTTTGGGTCCTTCTTCAGTTGATTC-3′
Table 1: Sequences of the primers used to amplify the msp 1 and msp 2 genes of P. falciparum isolates