Groups Challenge post challenge   0 days 7th days of Vaccination 15th days of Vaccination 7 days 14 days 21 days 28 days 35 days 42 days 49 days 56 days Group-A - 181.0 362.0 415.9 415.9 445.7 445.7 362.0 362.0 362.0 362.0 Group-B - 181.0 362.0 445.7 445.7 445.7 445.7 415.9 415.9 415.9 415.9 Group-C - 128.0 256.0 388.0 388.0 388.0 315.2 315.2 315.2 315.2 315.2 Group-D - - - 39.4 48.5 32.0 32.0 32.0 32.0 32.0 32.0

Table 2: Geometric mean Antibody titer by Indirect Haemagglutination (IHA) test against E. bovis in calves vaccinated with sonicated sporocyst vaccine (challenged). Group A: Supernatant from sonicated sporulated oocysts; Group B: Sediment from sonicated sporulated oocysts; Group C: Un-sonicated sporulated oocysts; Group D: Non-vaccinated group