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Figure 1: Genomic location of waaL and Tn5 insertion in waaL. (A) The genomic region where a cluster of waa genes are located. The locus tag (the prefix A936_ was omitted) and gene names were indicated. The waaL gene encodes a 402 aa protein. (B) Insertion site of the Ez-Tn5 transposon in the waaL gene. The transposon was inserted between the 2nd (A) and 3rd (T) nucleotide of the 298th codon (GAT/D) and generated a 9 bp direct repeat (underlined) flanking the Tn5 insert. |