Media used Growth Hours      Culture pH Opacity IOU (x109 org / ml) Average yield (Opacity IOU/ml) Purity Sterility Ex 1 Ex 2 Ex 3 Ex 1 Ex 2 Ex 3 B2 24 7.51 7.41 7.41 30 25 23 26.00 Passed Passed B2 36 7.90 7.82 7.62 50 48 45 47.66 Passed Passed B2 48 8.10 8.15 8.10 75 70 65 70.00 Passed Passed

Table 1b: Growth yield analysis of B. pertussis strain 509 at different growth hours in vortex mode Fermentor cultivation. Ex: Experiment, Org: Organisms, IOU: International opacity units.