Vaccine Sample Average weight gain gram(g)per mouse Days after Injection Mortality % of Control Test Results Day 1 Day 3 Day 7 Baffle culture -1.0 1.8 4.9 0 65 Passed Vortex Culture -0.7 2.0 5.2 0 75 Passed Saline Control 1.0 1.4 6.1 0 NA NA Standard Batch -1.0 1.6 5.4 0 65 Passed

Table 2: Toxicity/Mouse weight gain analysis of B. Pertussis 509 strain culture of Baffle/Vortex mode. g-Grams, NA-Not applicable, Number of Mice used per test: 10; Weight of Mice: 14-16 grams Dilution: half of a single human dose, Route of inoculation: Inraperitonial.