Figure 1a: Impact of a 24 h EOB treatment, with different concentrations ranging from 0.005% to 0.5%, on PAO1 biofilm formation. Biofilms were grown in the wells of a microplate, using the LB medium and increasing concentrations of EOB. In each well ECO was used as emulsifier in same concentrations related to EOB. Use of ECO at a maximal concentration of 0.5% did not cause any inhibition. Biofilm was quantified after a 24 h incubation using crystal violet. Experiments were carried out in triplicate (n=3) and error bars indicate the standard deviation from the mean.