Figure 1b: Fluorescence microscopic visualisation of PAO1 cell adhesion and biofilm formation on glass slides in different conditions. Adhesion and biofilm growth were performed in control condition, in presence of 0.1% ECO or in presence of 0.1% EOB emulsified with 0.1% ECO, according to the respective protocols. Attached cells and biofilms were stained with DAPI (28.5 μM) and observed at magnification x1000. Scale represents 8μm. Covered area fractions of adhered cells were obtained after treatment with IMAGEJ software. Experiments were carried out in triplicate and three images were taken pro slides. Error bars represent standard deviation from the mean (n=9) and ***P<0.001.