Figure 2:SRE-dependent effects of genistein on LDLR promoter activity. Luciferase activities of phLDLR-luc(-805+50) and its corresponding SRE deletion construct (A) and pSRE-luc construct (B) upon the treatment of 10 or 20 µM genistein (G10 µM or G20 µM) and DMSO (G0 µM) for 24 h. CHIP assay for binding of SREBP-2 to SRE regions of the human LDLR promoter region (C). The results are expressed as mean ± SD of at least three independent experiments. Different letters (a-c) represent significant differences (p<0.05) for the comparison of all groups by ANOVA (A and B) or two groups by student’s t-test (C).