Figure 4:JNK activation by genistein involves SREBP-2 processing and LDLR expression. Phosphorylated levels of JNK, p38 and ERK upon 10 µM genistein treatment for the indicated period of times (A) and quantification of p-JNK levels (B). LDLR and p-JNK protein levels with pre-treatment of SP600126 or vehicle DMSO (C) and quantification of LDLR protein levels (D). Protein levels of pre-SREBP-2 and nuclear SREBP-2 in cells treated with genistein for 6 or 24 h after 30 min incubation with SP600126 or DMSO (E). Luciferase activities of pLDLR-luc (-171 to +50) and pLDLRdelSRE-luc (-171 to +50) upon 24 h incubation of 10µM genistein (G10µM) or DMSO (G0µM). The results are expressed as mean ± SE of at least three independent experiments. Different letters (a-c) represent significant differences between all groups (p<0.05) by ANOVA.