Samples Quantities of samples HNO3
(mL)
H2O2
(mL)
Digestion program

Steps Time
(min)
Power
(W)
Temperature
(ºC)
References
Coffee powder 300 (mg) 7 1 1 5 750 120 45
(n=4)     2 3 750 120
      3 10 750 210
      4 15 750 210
Coffee infusion* 2 (mL) 5 1 1 5 400 80 46
(n=4)     2 5 400 120
      3 5 400 210
Rat livers
and diets
300 (mg) 6 2 1 3 250 130 46
(n=4)     2 5 630 130
      3 22 500 130
      4 15 0 130
*All infusion volumes were reduced to 1/4 of the initial volume.
Table 1: Conditions of digestion program used in coffee samples, rat livers and diets.