Group/ Treatment nº of rats chemicals form of coffee applied diet G1/commercial 10 DMH - CD G2/organic 10 DMH 5% infusion ORC 5%* G3/organic 10 DMH 10% infusion ORC 10% G4/organic 10 DMH 20% infusion ORC 20% G5/organic 10 DMH powder ORC 4% G6/conventional 10 DMH 5% infusion COC 5% G7/conventional 10 DMH 10% infusion COC 10% G8/conventional 10 DMH 20% infusion COC 20% G9/conventional 10 DMH powder COC 4% G10/commercial 10 EDTA - CD G11/organic 10 EDTA 20% infusion ORC 20% G12/organic 10 EDTA powder ORC 4% G13/conventional 10 EDTA 20% infusion RCC 20% G14/conventional 10 EDTA powder RCC 4%

DMH=1,2-dimetilhidrazine (40mg. Kg,-1 body weight), a promoter of preneoplastic lesions in the colon. EDTA 1,5% (w/v) in NaCl 0,9% (w/v) (vehicle). CD=commercial diet; COC=diet with organic coffee; COC=diet with conventional coffee.*Considering the infusion embedded the feed (incorporated 100 mL infusion/kg commercial diet).

Table 2: Experimental design for the 12-weeks experiment to investigate the hepatic mineral content in healthy and induced cancer animals (DMH), fed with these coffees.