Figure 4: Effects of EPK on serum lipid and cholesterol levels and on hepatic triglyceride and cholesterol in HFD-fed rats. (A) Triglyceride levels were measured by a triglyceride assay kit (AM157S-K, Asan PharmCo., Seoul, Korea). (B) Total cholesterol levels were measured by using a total cholesterol assay kit (AM202-K, Asan PharmCo., and Seoul, Korea). (C) The levels of high-density lipoprotein (HDL) and low-density lipoprotein (LDL) in serum were measured using cobas c 111 analyzer (Roche-Diagnostics, Indianapolis, IN, USA). (D) AI was calculated by employing the following equation: AI=(total cholesterol − HDL cholesterol)/HDL cholesterol. (E) The levels of triglyceride in liver were measured by Biochemistry Analyzer. (F) The levels of total cholesterol in liver were measured by Biochemistry Analyzer. Values with the different superscript letters indicate statistical significance (P<0.05) between groups by Duncan’s multiple range tests.