| Enzyme Fraction |
EA (U) |
TP (mg) |
Specific EA (U/mg) |
PF |
% Yield |
| Crude homogenate |
0.638 ± 0.04 |
0.880 ± 0.04 |
0.725 ± 0.03 |
1.00 |
100 |
| Centrifuged at 32000 g |
0.513 ± 0.02 |
0.098 ± 0.02 |
5.235 ± 0.03 |
7.22 |
80.4 |
| 80% (NH4)2SO4 |
0.324 ± 0.02 |
0.043 ± 0.02 |
7.535 ± 0.02 |
10.39 |
50.8 |
| Ultra-filtration |
0.287 ± 0.01 |
0.032 ± 0.02 |
8.969 ± 0.02 |
12.37 |
45.0 |
| Dialysis |
0.284 ± 0.01 |
0.026 ± 0.02 |
10.923 ± 0.03 |
15.07 |
44.5 |
EA: Enzyme activity; TP: Total protein; PF: Purification fold. Values are mean ± SD of six (n=6) determinations. |