Hydrolysis timeĀ  (hour) Thermolysin Alcalase
DH Peptide content DH Peptide content
(%) (mg/ml) (%) (mg/ml)
0.5 27.41 2.62 ± 0.17d 26.85 2.03 ± 0.11d
1 47.3 4.78 ± 0.15c 34.19 3.35 ± 0.07c
2 76.29 7.92 ± 0.03b 63.49 6.53 ± 0.23b
3 88.01 9.20 ± 0.25a 80.66 8.40 ± 0.13a
4 92.58 9.70 ± 0.28a 85.3 8.90 ± 0.27a
abcdDifferent lowercase letters indicate significant differences (P<0.05) between samples (n=2).
Table 1: Effect of hydrolysis time and different enzymes (Thermolysin and Alcalase) on degree of hydrolysis and peptide content of O. niloticus protein hydrolysate (n=2).