Effect

Samples	Size interval	DPPH activity (%)		ABTS activity (%)
Samples	Size interval	Thermolysin	Alcalase	Thermolysin	Alcalase
Native protein	-	12.31 ± 2.03^d		14.81 ± 0.69^d
Hydrolysates After hydrolysis.	1	38.98 ± 0.70^b	38.89 ± 0.71^b	44.78 ± 0.06^b	40.41 ± 0.16^b
(chosen time interval (hour))	2	34.70 ± 0.52^c	31.09 ± 0.66^c	37.82 ± 0.09^c	34.64 ± 0.13^c
After ultrafiltration	10 kDa	42.51 ± 0.67^a	44.30 ± 0.01^a	54.00 ± 0.01^a	44.60 ± 0.04^a
(diff. membrane cut-off (MWCO))	3kDa	14.70 ± 0.01^d	11.50 ± 0.06^e	20.00 ± 0.07^d	13.60 ± 0.03^e

^abcdeDifferent lowercase letters indicate significant differences (P<0.05) between samples.

Table 2: Effect of hydrolysis time and low molecular weight (after one hour hydrolysis of protein) with different enzymes on anti-oxidant activity of O. niloticus protein hydrolysate with DPPH and ABTS assay (n=3).