Figure 3: (A) Effects of Hcy treatment for different time lengths on mRNA and protein expression of MMP-2. VECs were incubated with 100 mmol/L Hcy for 24 h, 48 h, 72 h. The mRNA of MMP-2 was tested by Real-time PCR (Figure 3A), and MMP-2 protein was examined by western blot assay. (B) A: x ±s, n=9, *P<0.01 vs. N (0 h); and P<0.01 vs. T1 (24 h), B: x ±s, n=5, *P<0.01 vs. control N (0 h); #P<0.01 vs. T1 (24 h).