Figure 15: HPLC profile of a methanolic extract of variety C of Piper betle. Chromatogram of crude Sample C prepared from methanolic extract of P. betle L. leaf, on analytical RP-HPLC in isocratic mode using acetonitrile and water (1:1) as mobile phase and C18 column as stationary phase, at a flow rate of 0.5ml/min. Six peaks were identified at 254nm and their retention times were 5.21, 6.08, 6.42, 7.01, 7.44 and 7.97 min respectively.