Figure 17: HPLC profile of a methanolic extract of variety E of Piper betle. Chromatogram of crude Sample E prepared from methanolic extract of P. betle L. leaf, on analytical RP-HPLC in isocratic mode using acetonitrile and water (1:1) as mobile phase and C18 column as stationary phase, at a flow rate of 0.5 ml/min. Only two peaks were found at 254 nm and their retention times were 6.73 and 7.16 min respectively.