Figure 19: HPLC profile of a methanolic extract of variety G of Piper betle. Chromatogram of crude Sample G prepared from methanolic extract of P. betle L. leaf, on analytical RP-HPLC in isocratic mode using acetonitrile and water (1:1) as mobile phase and C18 column as stationary phase, at a flow rate of 0.5 ml/min. Four peaks were identified at 254 nm and their retention times were 15.04, 16.19, 17.79 and 18.79 min respectively.