Figure 20: HPLC profile of a methanolic extract of variety H of Piper betle. Chromatogram of crude Sample H prepared from methanolic extract of P. betle L. leaf, on analytical RP-HPLC in isocratic mode using acetonitrile and water (1:1) as mobile phase and C18 column as stationary phase, at a flow rate of 0.5 ml/min. Three peaks were identified at 254 nm and their retention times were 6.40, 6.70 and 9.42 min respectively.