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Figure 6: ROS generation assay in RBE cells treated with 200 μM EPS, 160 μM PA or a combination of 200 μM EPS and 160 μM PA for 24 hrs. Images (A) were acquired using a Nikon Te2000 microscope (magnification 200×). The fluorescence intensity was quantified and percentage in ROS production of treated cells relative to that of vehicle control cells (B) was calculated. Data from at least 3 randomly selected images are expressed as mean ± SD (*, P<0.05 versus **). |