Figure 2: Chromatogram of the permeate of the substrate addition reaction. Arabinan (5%) hydrolysis by viscozyme (54 mg protein ml-1) in a membrane reactor fitted with 5 Kda MWCO. Arabinan was mixed with viscozyme and incubated at 35°C for 6 h and substrate was added each hour as required. Samples of permeate were taken in each hour and run through Bio-Gel P2 column at a flow rate of 2.2 ml min-1 then detected by a refractive index.