Visual score of DNA damage

Treatment	Number of animals	No. of cells			Class¥ of comet				DNA damaged cells (%)
		*Analyzed ()**	Total comets		0	1	2	3
		*Analyzed ()**	Individuals	Total	0	1	2	3
Control	3	300	(7+6+7)	20	280	16	4	0	6.7
STZ	3	300	(25+24+24)	73	227	22	24	27	24.3
STZ+Insulin	3	300	(14+13+15)	42	258	13	16	13	14
STZ+50 mg costus	3	300	(19+20+21)	60	240	20	19	21	20
STZ+100 mg costus	3	300	(18+17+18)	53	247	16	18	19	17.7
STZ+ 150 costus	3	300	(14+16+15)	45	255	19	13	13	15
STZ+50mg nanocostus	3	300	(15+16+16)	47	253	14	17	16	15.7
STZ+100mg nanocostus	3	300	(11+13+12)	36	264	11	13	12	12
STZ+150mg nanocostus	3	300	(12+11+12)	35	265	11	12	12	11.7

Table 1: Visual score of DNA damage in blood samples of male rats exposed to streptozotocin (STZ) and/or costus or nanocostus extracts using comet assay. ¥: Class 0 = no tail; 1 = tail length < diameter of nucleus; 2 = tail length between 1X and 2X the diameter of nucleus; and 3 = tail length > 2X the diameter of nucleus. (*): No of cells analyzed were 100 per animal.