Figure 4: Plasma ORAC values on day 21 in diabetic and control rats, treated or not with extracts from leaf, root and seed of Zyziphus lotus L. for3 weeks. ORAC activity was determined by employing a fluorescent protein, i.e., allophycocyanin (APC), as described in the Materials and Methods. The absorbance decay of APC was measured at 598 nm (excitation) and 651 nm (emission) every 5 min, at 37°C, using a spectrofluorometer until the disappearance of fluorescence. The ORAC value of each plasma sample was calculated by measuring the net protection area under the quenching curve of APC with trolox, sample or blanc. Each value represents the mean ± SEM (n=6). NS=insignificant difference as compared to diabetic rats.