Target allergen Limit of detection (ppm) Quantification range (ppm) Results expressed as ppm of Extraction buffer,  pH Supplier and kit name
Casein 1 1.6-25.6 Casein Carbonate/bicarbonate
buffer at pH 9.6
Neogen (BioKits)
2.5 2.5-15 Non fat dried milk PBS, pH 7.4 and specific allergen extraction additive Neogen (Veratox)
Soy protein 0.3 1.25-20 Soy protein Tris buffer containing   sodium chloride and gelatine, pH 8.2 Neogen (BioKits)
2.5 2.5-25 Soy flour PBS, pH 7.4 and allergen extraction additive Neogen (Veratox)
Egg 0.1 0.5-10 Egg white protein Tris buffer containing   sodium chloride and gelatine, pH 8.2 Neogen (BioKits)
2.5 2.5-25 Whole dried egg PBS, pH 7.4 and allergen extraction additive Neogen (Veratox)
Table 2: ELISA test kits employed.