Sample Description % antigen 1 recovered Homogenized supernatant (12 L) 100 Homogenized pellet (12 L) 0 Clarified permeate during 5-fold concentration of homogenate (9.5 L) 75 Diafiltered permeate (4x) (6L) 22 Unclarified Retentate (0.2 L) 0.5

Table 2: The recovery of antigen after clarification using cross-flow filtration.