A.Digestion of pSET152-otrB. Lane 1: marker DL15000; lane 2: pSET152-
otrB digested with NdeI; lane 3: pSET152-otrB digested with XbaI and NdeI.
B. PCR identification of SRI mutants. Lanes 1–7: SRI-A mutants; lane 8–9:
positive control(750bp for apramycin resistance gene); lane 10–16: SRI-B mutants;
lane 17: marker III.
C. Site-specific integration of otrA in the SRI genome.