Run No. |
CCD Experimental Design matrix (Coded and real values) |
Experimental |
Predicted Lipase
activity
|
Predicted
Cell mass |
| |
x1 |
x2 |
x3 |
Cell massa
(g l-1) |
Lipase activityb
(U ml-1) |
Protease activityc
(U ml-1) |
RSM
(U ml-1) |
ANN
(U ml-1) |
RSM
(g l-1) |
ANN
(g l-1) |
|
1 |
-1 (28) |
-1 (5) |
-1 (120) |
4.20 |
2.15 |
0.95 |
1.969 |
2.15 |
4.432 |
4.2 |
|
2 |
1 (32) |
-1 (5) |
-1 (120) |
6.56 |
3.00 |
1.65 |
3.36 |
3.0 |
6.807 |
6.56 |
|
3 |
-1 (28) |
1 (6.5) |
-1 (120) |
3.15 |
2.20 |
1.05 |
2.052 |
2.2 |
3.159 |
3.15 |
|
4 |
1 (32) |
1 (6.5) |
-1 (120) |
5.82 |
3.8 |
0.45 |
3.518 |
3.8 |
5.499 |
5.82 |
|
5 |
-1 (28) |
-1 (5) |
1 (160) |
3.30 |
1.90 |
0.85 |
1.975 |
1.9 |
3.772 |
3.3 |
|
6 |
1 (32) |
-1 (5) |
1 (160) |
3.65 |
2.25 |
1.95 |
2.191 |
2.25 |
3.792 |
3.65 |
|
7 |
-1 (28) |
1 (6.5) |
1 (160) |
3.12 |
2.65 |
0.65 |
2.083 |
2.65 |
3.024 |
3.12 |
|
8 |
1 (32) |
1 (6.5) |
1 (160) |
3.09 |
2.40 |
1.26 |
2.374 |
2.4 |
3.008 |
3.09 |
|
9 |
-1.682 (26.6) |
0 (5.75) |
0 (140) |
2.60 |
1.20 |
0.55 |
1.588 |
1.2 |
2.305 |
2.6 |
|
10 |
1.682 (33.3) |
0 (5.75) |
0 (140) |
4.21 |
3.10 |
2.15 |
3.003 |
3.1 |
4.29 |
4.21 |
|
11 |
0 (30) |
-1.682 (4.46) |
0 (140) |
5.81 |
2.60 |
0.88 |
2.383 |
2.6 |
5.232 |
5.81 |
|
12 |
0 (30) |
1.682 (7.01) |
0 (140) |
3.14 |
2.10 |
0.45 |
2.607 |
2.1 |
3.503 |
3.14 |
|
13 |
0 (30) |
0 (5.75) |
-1.682 (106.4) |
6.41 |
3.20 |
1.65 |
3.249 |
3.2 |
6.382 |
6.41 |
|
14 |
0 (30) |
0 (5.75) |
1.682 (173.6) |
3.92 |
2.05 |
1.65 |
2.292 |
2.05 |
3.733 |
3.92 |
|
15 |
0 (30) |
0 (5.75) |
0 (140) |
5.20 |
4.10 |
2.15 |
3.841 |
3.925 |
5.139 |
5.133 |
|
16 |
0 (30) |
0 (5.75) |
0 (140) |
4.92 |
3.70 |
1.95 |
3.841 |
3.925 |
5.139 |
5.133 |
|
17 |
0 (30) |
0 (5.75) |
0 (140) |
4.56 |
4.20 |
1.89 |
3.841 |
3.925 |
5.139 |
5.133 |
18 |
0 (30) |
0 (5.75) |
0 (140) |
5.95 |
3.85 |
2.30 |
3.841 |
3.925 |
5.139 |
5.133 |
19 |
0 (30) |
0 (5.75) |
0 (140) |
4.85 |
3.90 |
1.65 |
3.841 |
3.925 |
5.139 |
5.133 |
20 |
0 (30) |
0 (5.75) |
0 (140) |
5.32 |
3.80 |
2.01 |
3.841 |
3.925 |
5.139 |
5.133 |
I1: Temperature; x2: Initial pH; x3: Agitation speed
a,b,cThe maximum values of cell mass, lipase activity and protease activity respectively and they are the mean values of triplicates. 250 ml Erlenmeyer flask containing 100
ml production medium (constant) was incubated in an orbital shaker (variable) for the fermentation period of 48 hrs. |
